New species and record of Dodecaceria (Annelida: Cirratulidae) the Biological Reserve of Rocas Atoll, Brazil, the only atoll in the South Atlantic Ocean.

The polychaete Family Cirratulidae is one of the most abundant and diverse groups of Annelida, although it remains poorly known worldwide. Dodecaceria Ørsted, 1843 is one of the least described genera of Cirratulidae. The present report is the first taxonomic study of the genus Dodecaceria for the Brazilian coast. Cirratulidae were collected at Rocas Atoll, the first Brazilian marine protected area and the only atoll in the South Atlantic Ocean. We described one new species, Dodecaceria zelinhae n. sp., and a new record of D. dibranchiata Blake & Dean, 2019, previously only known from Panama. The new species is distinguished from other Dodecaceria species by having lateral tentacles, a smooth peristomium, 3-5 pairs of branchiae, hooks from chaetiger 11 in notopodia and 9 in neuropodia. Dodecaceria dibranchiata, a Caribbean species, is here recorded for the first time in the South Atlantic Ocean.

Monsters reveal patterns: bifurcated annelids and their implications for the study of development and evolution.

During recent decades, the study of anatomical anomalies has been of great relevance for research on development and its evolution. Yet most animal groups have never been studied under this perspective. In annelids, one of the most common and remarkable anomalies is anteroposterior axis bifurcation, that is animals that have two or more heads and/or tails. Bifurcated annelids were first described in the 18th century and have been occasionally reported since then. However, these animals have rarely been considered other than curiosities, one-off anomalies, or monsters, and a condensed but comprehensive analysis of this phenomenon is lacking. Such an analysis of the existing knowledge is necessary for addressing the different patterns of annelid bifurcation, as well as to understand possible developmental mechanisms behind them and their evolution. In this review we summarize reports of annelid bifurcation published during the last 275 years and the wide variety of anatomies they present. Our survey reveals bifurcation as a widespread phenomenon found all over the annelid tree. Moreover, it also shows that bifurcations can be classified into different types according to anatomy (lateral versus dorsoventral) or developmental origin (embryonic versus postembryonic, the latter occurring in relation to regeneration, reproduction, or growth). Regarding embryos, three different types of bifurcation can be found: conjoined twins (in clitellates); Janus embryos (two posterior ends with a single head which shows duplicated structures); and duplicitas cruciata embryos (with anterior and posterior bifurcation with a 90° rotation). In adults, we show that while lateral bifurcation can result in well-integrated phenotypes, dorsoventral bifurcation cannot since it requires the discontinuity of at least some internal organs. The relevance of this distinction is highlighted in the case of the Ribbon Clade, a group of syllid annelids in which some species reproduce by collateral and successive gemmiparity (which involves dorsoventral bifurcation), while others grow by branching laterally. Although most known cases of bifurcation came from accidental findings in the wild or were unintentionally produced, experimental studies resulting in the induction of bifurcation of both embryos and adults are also reviewed. In embryos, these experimental studies show how mechanical or chemical disruption of the zygote can result in bifurcation. In adults, the ventral nervous system and the digestive tract seem to play a role in the induction of bifurcation. Based on the reviewed evidence, we argue that the long-forgotten study of annelid developmental anomalies should be incorporated into the growing field of annelid EvoDevo and examined with modern techniques and perspectives.

Integrative anatomical study of the branched annelid Ramisyllis multicaudata (Annelida, Syllidae).

The sponge-dwelling Syllidae Ramisyllis multicaudata and Syllis ramosa are the only annelid species for which a branched body with one head and multiple posterior ends is known. In these species, the head is located deep within the sponge, and the branches extend through the canal system of their host. The morphology of these creatures has captivated annelid biologists since they were first discovered in the late XIXth century, and their external characteristics have been well documented. However, how their branched bodies fit within their symbiotic host sponges and how branches translate into internal anatomy has not been documented before. These features are crucially relevant for understanding the body of these animals, and therefore, the aim of this study was to investigate these aspects. In order to assess these questions, live observation, as wells as histology, immunohistochemistry, micro-computed tomography, and transmission electron microscopy techniques were used on specimens of R. multicaudata. By using these techniques, we show that the complex body of R. multicaudata specimens extends greatly through the canal system of their host sponges. We demonstrate that iterative external bifurcation of the body is accompanied by the bifurcation of the longitudinal organ systems that are characteristic of annelids. Additionally, we also highlight that the bifurcation process leaves an unmistakable fingerprint in the form of newly-described "muscle bridges." These structures theoretically allow one to distinguish original and derived branches at each bifurcation. Last, we characterize some of the internal anatomical features of the stolons (reproductive units) of R. multicaudata, particularly their nervous system. Here, we provide the first study of the internal anatomy of a branched annelid. This information is not only crucial to deepen our understanding of these animals and their biology, but it will also be key to inform future studies that try to explain how this morphology evolved.

Regeneration mechanisms in Syllidae (Annelida).

Syllidae is one of the most species-rich groups within Annelida, with a wide variety of reproductive modes and different regenerative processes. Syllids have striking ability to regenerate their body anteriorly and posteriorly, which in many species is redeployed during sexual (schizogamy) and asexual (fission) reproduction. This review summarizes the available data on regeneration in syllids, covering descriptions of regenerative mechanisms in different species as well as regeneration in relation to reproductive modes. Our survey shows that posterior regeneration is widely distributed in syllids, whereas anterior regeneration is limited in most of the species, excepting those reproducing by fission. The latter reproductive mode is well known for a few species belonging to Autolytinae, Eusyllinae, and Syllinae. Patterns of fission areas have been studied in these animals. Deviations of the regular regeneration pattern or aberrant forms such as bifurcated animals or individuals with multiple heads have been reported for several species. Some of these aberrations show a deviation of the bilateral symmetry and antero-posterior axis, which, interestingly, can also be observed in the regular branching body pattern of some species of syllids.

Patterns of mollusc distribution in mangroves from the São Marcos Bay, coast of Maranhão State, Brazil / Padrões de distribuição de moluscos em manguezais da Baía de São Marcos, costa do Estado do Maranhão, Brasil

The diversity and distribution of molluscs from the Amazon Coast of Maranhão State, Brazil, are poorly understood. The aim of this study was to investigate how molluscs in two mangrove creeks (Buenos Aires and Tronco) at the São Marcos Bay, coast of the Maranhão State, respond to spatial and temporal variations in the environment. Sampling was performed in the intertidal area along three zones established using a straight line transect of 100 m. Abiotic variables of water and sediment were measured at each creek. We found 5,912 specimens belonging to 23 species and 15 families of epifaunal and infaunal molluscs. The patterns of their distribution in the two creeks were different. Salinity, dissolved oxygen, and rainfall were the main variables that affected the temporal distribution of molluscs. We found low species richness in the overall mollusc composition. Diversity in the Buenos Aires Creek was lower than that observed in the Tronco Creek, possibly because of activities of a port located in proximity to the former. The spatial distribution of molluscs along the zones followed an abundance and diversity gradient, mainly influenced by exposure time during low tide. Port activities may influence the patterns of mollusc distribution in the surrounding mangroves, and we thus highlight the importance of management and monitoring of these areas.

A diversidade e distribuição de moluscos na Costa Amazônica do Estado do Maranhão, Brasil, são pobremente conhecidas. O objetivo deste estudo foi investigar como os moluscos em dois igarapés de manguezal (Buenos Aires e Tronco) na Baía de São Marcos, costa do Estado do Maranhão, respondem a variações espaciais e temporais no ambiente. A amostragem foi conduzida no entre-marés ao longo de três zonas estabelecidas a partir de um transecto em linha reta de 100 m. As variáveis abióticas de água e sedimento foram medidas para cada igarapé. Foram contabilizados 5.912 espécimes pertencentes a 23 espécies e 15 famílias na epifauna e endofauna. Os padrões de distribuição de moluscos foram distintos entre os dois igarapés. Salinidade, oxigênio dissolvido e precipitação foram as principais variáveis que influenciaram a distribuição temporal dos moluscos. Observou-se baixa riqueza de espécies na composição geral de moluscos. A diversidade no igarapé Buenos Aires foi menor do que a observada no igarapé Tronco, possivelmente por causa das atividades portuárias nas proximidades do primeiro. A distribuição espacial vertical de moluscos ao longo das zonas seguiu um gradiente de abundância e diversidade influenciado principalmente pelo tempo de exposição durante a maré baixa. As atividades portuárias podem estar influenciando os padrões de distribuição de moluscos nos manguezais do entorno, portanto destacamos a importância do manejo e monitoramento dessas áreas.